TY - JOUR
T1 - Preliminary study of the effect of hemolysis on platelet aggregation through microscopic observation under physiological shear flow
AU - Watanabe, Nobuo
AU - Kawada, Naoki
N1 - Funding Information:
Acknowledgements The authors appreciate Professor John F. Fraser, Prince Charles Hospital Brisbane, Dr. Michael J. Simmonds, Griffith University, and Dr. Jarod T. Horobin, Perth Blood Institute, for their special introduction and scientific discussion about clinical research topics, which motivated us to begin this study. Some of the content of this paper was presented by NK at the annual conference of the Japan Biorheology Society, July 2021. This study was financially supported by KAKENHI Grant No. 20K12609 (Principal Investigator: NW).
Funding Information:
The authors appreciate Professor John F. Fraser, Prince Charles Hospital Brisbane, Dr. Michael J. Simmonds, Griffith University, and Dr. Jarod T. Horobin, Perth Blood Institute, for their special introduction and scientific discussion about clinical research topics, which motivated us to begin this study. Some of the content of this paper was presented by NK at the annual conference of the Japan Biorheology Society, July 2021. This study was financially supported by KAKENHI Grant No. 20K12609 (Principal Investigator: NW).
Publisher Copyright:
© Japanese Society of Biorheology 2021.
PY - 2021
Y1 - 2021
N2 - Hemolysis during mechanical circulatory support has been suggested as a possible trigger for non-hemorrhagic stroke. The purpose of this study was to investigate the hypothesis that slight hemolysis enhances thrombus formation through platelet aggregation under physiological shear stress in humans. Using sodium citrate-treated porcine whole blood from a slaughterhouse, platelet suspensions with a constant platelet density of 20.5 ± 1.3 × 103 cells/μL with three plasma free hemoglobin (pfHb) concentrations (44.9 ± 15.0, 74.3 ± 18.3, and 130.6 ± 20.3 mg/dL) were prepared through centrifugation. These suspensions were exposed to a physiological shear rate up to 200 (1/s) using our custom-built shear chamber for 0, 5, 10, and 15 min. After exposure to each shear load, microscopic image acquisition was performed and the images were analyzed to count the number of aggregated platelets. It was observed that platelet aggregation increased in an exposure timedependent manner in all suspension fluids. In addition, the samples with the highest mean pfHb concentration of 130.6 mg/dL showed 1.23-and 1.28-fold numerically greater aggregation than those with a pfHb concentration of 74.3 and 44.9 mg/dL, respectively. The Wilcoxon signed-rank sum test showed p-values of 0.028 and 0.047 between 5 vs. 10 min and 10 vs. 15 min under the lowest pfHb concentration, respectively, and a p-value of 0.028 between 0 vs. 5 min under the medium pfHb concentration. However, there was no significant difference in aggregation according to pfHb concentration at the same exposure time. From these results, our data suggest that hemolysis might enhance platelet aggregation under physiological shear conditions.
AB - Hemolysis during mechanical circulatory support has been suggested as a possible trigger for non-hemorrhagic stroke. The purpose of this study was to investigate the hypothesis that slight hemolysis enhances thrombus formation through platelet aggregation under physiological shear stress in humans. Using sodium citrate-treated porcine whole blood from a slaughterhouse, platelet suspensions with a constant platelet density of 20.5 ± 1.3 × 103 cells/μL with three plasma free hemoglobin (pfHb) concentrations (44.9 ± 15.0, 74.3 ± 18.3, and 130.6 ± 20.3 mg/dL) were prepared through centrifugation. These suspensions were exposed to a physiological shear rate up to 200 (1/s) using our custom-built shear chamber for 0, 5, 10, and 15 min. After exposure to each shear load, microscopic image acquisition was performed and the images were analyzed to count the number of aggregated platelets. It was observed that platelet aggregation increased in an exposure timedependent manner in all suspension fluids. In addition, the samples with the highest mean pfHb concentration of 130.6 mg/dL showed 1.23-and 1.28-fold numerically greater aggregation than those with a pfHb concentration of 74.3 and 44.9 mg/dL, respectively. The Wilcoxon signed-rank sum test showed p-values of 0.028 and 0.047 between 5 vs. 10 min and 10 vs. 15 min under the lowest pfHb concentration, respectively, and a p-value of 0.028 between 0 vs. 5 min under the medium pfHb concentration. However, there was no significant difference in aggregation according to pfHb concentration at the same exposure time. From these results, our data suggest that hemolysis might enhance platelet aggregation under physiological shear conditions.
KW - Hemolysis
KW - Physiological shear stress
KW - Platelet aggregation
KW - Shear chamber
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U2 - 10.17106/jbr.35.62
DO - 10.17106/jbr.35.62
M3 - Article
AN - SCOPUS:85124334687
SN - 1867-0466
VL - 35
SP - 62
EP - 67
JO - Journal of Biorheology
JF - Journal of Biorheology
IS - 2
ER -