TY - GEN
T1 - Regeneratable surface acoustic wave (SAW) immunosensor for monitoring of physiological information
AU - Toma, Koji
AU - Oishi, Koki
AU - Arakawa, Takahiro
AU - Mitsubayashi, Kohji
AU - Yoshimura, Naoyuki
AU - Yatsuda, Hiromi
N1 - Funding Information:
ACKNOWLEDGMENT This work was supported by Japan Radio Co. Ltd., the Japan Society for the promotion of Science (JSPS) Grant-in-Aid for Scientific Research System, the Japan Science and Technology Agency (JST), and the Ministry of Education, Culture, Sports, Science and Technology (MEXT).
Publisher Copyright:
© 2017 IEEE.
PY - 2018/2/27
Y1 - 2018/2/27
N2 - A reusable immunosensor that employed surface acoustic wave (SAW) was developed for monitoring of physiological information. The SAW immunosensor was fabricated by modifying a propagation area of the SAW device with a self-assembled monolayer of pH tolerant protein, ORLA85, which allowed repeating of surface regeneration by changing pH, and thereby rapid and repetitive (semi-continuous) measurement of analyte concentration. In experiment, initially a SAW device used for the sensor was characterized using a series of solution with various viscosity, and results showed a good correlation between phase change and solution viscosity from 0.88 to 1.7 mPa-s. In addition, performance of developed SAW immunosensor was evaluated using mouse IgG and anti-mouse IgG as model capturing molecule and analyte. It revealed that dynamic range of the immunosensor was 0.16-100 μg/mL with a measurement time of 20 minutes, and semi-continuous measurement was possible with a high reproducibility represented by a coefficient of variation of 3.4% for five repetitive measurement. These demonstrated that SAW immunosensor holds a huge potential for application to monitoring of time course of biomolecules which reflect physiological conditions.
AB - A reusable immunosensor that employed surface acoustic wave (SAW) was developed for monitoring of physiological information. The SAW immunosensor was fabricated by modifying a propagation area of the SAW device with a self-assembled monolayer of pH tolerant protein, ORLA85, which allowed repeating of surface regeneration by changing pH, and thereby rapid and repetitive (semi-continuous) measurement of analyte concentration. In experiment, initially a SAW device used for the sensor was characterized using a series of solution with various viscosity, and results showed a good correlation between phase change and solution viscosity from 0.88 to 1.7 mPa-s. In addition, performance of developed SAW immunosensor was evaluated using mouse IgG and anti-mouse IgG as model capturing molecule and analyte. It revealed that dynamic range of the immunosensor was 0.16-100 μg/mL with a measurement time of 20 minutes, and semi-continuous measurement was possible with a high reproducibility represented by a coefficient of variation of 3.4% for five repetitive measurement. These demonstrated that SAW immunosensor holds a huge potential for application to monitoring of time course of biomolecules which reflect physiological conditions.
KW - Component
KW - Immunosensor
KW - Monitoring
KW - Surface acoustic wave
KW - Surface regeneration
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U2 - 10.1109/ICSensT.2017.8304450
DO - 10.1109/ICSensT.2017.8304450
M3 - Conference contribution
AN - SCOPUS:85045614861
T3 - Proceedings of the International Conference on Sensing Technology, ICST
SP - 1
EP - 4
BT - 2017 11th International Conference on Sensing Technology, ICST 2017
PB - IEEE Computer Society
T2 - 11th International Conference on Sensing Technology, ICST 2017
Y2 - 4 December 2017 through 6 December 2017
ER -