Size of heparin-imprinted nanoparticles reflects the matched interactions with the target molecule

Yasuo Yoshimi, Daichi Oino, Hirofumi Ohira, Hitoshi Muguruma, Ewa Moczko, Sergey A. Piletsky

研究成果: Article査読

10 被引用数 (Scopus)


It has been shown that the faradic current at an electrode grafted with molecularly imprinted polymer (MIP) is sensitive to the specific target molecule used as the template. This phenomenon is applicable to sensors with very high selectivity, but the sensing mechanism is still a black box. We investigated the size sensitivity of nanoparticles of molecularly imprinted polymers (MIP-NPs) to a specific interaction for determination of the mechanism of the gate effect and its feasibility for new applications. Nanoparticles of poly(methacryloxy ethyl trimethylammonium chloride-co-acrylamide-co-methylenebisacrylamide) imprinted with heparin immobilized on glass beads were synthesized. The diameter of the MIP-NPs of heparin was increased by the presence of the heparin template but was insensitive to chondroitin sulfate C (CSC), the analogue of heparin. The high selectivity of the MIP-NPs was consistent with the selectivity of electrodes grafted with a heparin-imprinted polymer in our previous studies. The quartz crystal microbalance probes immobilizing heparin or CSC were sensitive to MIP-NPs, which indicates that the binding ability of MIP-NP does not discriminate between the template and other glycosaminoglycans. These results indicate that the size of the MIP-NP is sensitive to the matched binding with the template through the imprinted cavity.

ジャーナルSensors (Switzerland)
出版ステータスPublished - 2019 5月 2

ASJC Scopus subject areas

  • 分析化学
  • 情報システム
  • 生化学
  • 原子分子物理学および光学
  • 器械工学
  • 電子工学および電気工学


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