Visualization of mechanical stress-mediated Ca2+ signaling in the gut using intravital imaging

Yoshiko Aihara, Yota Fukuda, Akiyoshi Takizawa, Naomi Osakabe, Tomomi Aida, Kohichi Tanaka, Soichiro Yoshikawa, Hajime Karasuyama, Takahiro Adachi

研究成果: Article査読

2 被引用数 (Scopus)


Mechanosensory systems have been implicated in the maintenance of gut homeostasis, but details on the related mechanisms are scarce. Recently, we generated a conditional Ca2+ biosensor yellow cameleon 3.60 (YC3.60)-expressing transgenic mouse model and established a five-dimensional (5D; x, y, z, time, and Ca2+) intravital imaging system for investigating lymphoid tissues and enteric epithelial cell responses. To validate this gut-sensing system, we visualized responses of enteric nervous system (ENS) cells in Nestin-Cre/YC3.60flox mice with specific YC3.60 expression. The ENS, including the myenteric (Auerbach’s) and submucous (Meissner’s) plexuses, could be visualized without staining in this mouse line, indicating that the probe produced sufficient fluorescent intensity. Furthermore, the myenteric plexus exhibited Ca2+ signaling during peristalsis without stimulation. Nerve endings on the surface of enteric epithelia also exhibited Ca2+ signaling without stimulation. Mechanical stress induced transient salient Ca2+ flux in the myenteric plexus and in enteric epithelial cells in the Nestin-Cre/YC3.60 and the CAG-Cre/YC3.60 lines, respectively. Furthermore, the potential TRPM7 inhibitors were shown to attenuate mechanical stress-mediated Ca2+ signaling. These data indicate that the present intravital imaging system can be used to visualize mechanosensory Ca2+ signaling in ENS cells and enteric epithelial cells.

ジャーナルBioscience of Microbiota, Food and Health
出版ステータスPublished - 2020

ASJC Scopus subject areas

  • 食品科学
  • 微生物学
  • 免疫学
  • 応用微生物学とバイオテクノロジー
  • 消化器病学


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